Physiological Signaling and Structure of the HGF Receptor MET

The \u201chepatocyte growth factor\u201d also known as \u201cscatter factor\u201d, is a multifunctional cytokine with the peculiar ability of simultaneously triggering epithelial cell proliferation, movement and survival. The combination of those proprieties results in the induction of an epithelial to mesenchymal transition in target cells, fundamental for embryogenesis but also exploited by tumor cells during metastatization. The hepatocyte growth factor receptor, MET, is a proto-oncogene and a prototypical transmembrane tyrosine kinase receptor. Inhere we discuss the MET molecular structure and the hepatocyte growth factor driven physiological signaling which coordinates epithelial proliferation, motility and morphogenesis

Radio recombination lines from obscured quasars with the SKA

We explore the possibility of detecting hydrogen radio recombination lines from 0 < z < 10 quasars. We compute the expected Hnalpha flux densities as a function of absolute magnitude and redshift by considering (i) the range of observed AGN spectral indices from UV to X-ray bands, (ii) secondary ionizations from X-ray photons, and (iii) stimulated emission due to nonthermal radiation. All these effects are important to determine the line fluxes. We find that the combination of slopes: alpha_X,hard = -1.11, alpha_X,soft = -0.7, alpha_EUV = -1.3, alpha_UV = -1.7, maximizes the expected flux, f_Hnalpha = 10 microJy for z = 7 quasars with M_AB = -27 in the n = 50 lines; allowed SED variations produce variations by a factor of 3 around this value. Secondaries boost the line intensity by a factor of 2 to 4, while stimulated emission in high-z quasars with M_AB = -26 provides an extra boost to RRL flux observed at nu = 1 GHz if recombinations arise in HII regions with T_e = 10^3-5 K, n_e = 10^3-5 cm^-3. We compute the sensitivity required for a 5sigma detection of Hnalpha lines using the SKA, finding that the SKA-MID could detect sources with M_AB < -27 (M_AB < -26) at z < 8 (z < 3) in less than 100 hrs of observing time. These observations could open new paths to searches for obscured SMBH progenitors, complementing X-ray, optical/IR and sub-mm surveys.Comment: 11 pages, 9 figures; to be published in Monthly Notices of the Royal Astronomical Society Main Journa

experimental characterization of the 3d linear viscoelastic behavior of cold recycled bitumen emulsion mixtures

Cold mixtures with bitumen emulsion are produced at ambient temperature, leading to substantial reductions of energy consumption and atmospheric emissions. In cold recycling applications, cement is normally used to improve the mixture performance. Thus, the rheological behavior of cold recycled mixtures is different from that of conventional hot mixtures because it is due to the interaction of fresh bitumen, aged bitumen and cementitious bonds. In this study, we investigated the three-dimensional (3D) linear viscoelastic (LVE) behavior of a cement-bitumen treated material (CBTM) mixture fabricated using bitumen emulsion and cement. For comparison, we also investigated the 3D LVE behavior of hot-mix asphalt containing 25% of reclaimed asphalt and fabricated using polymer-modified binder. Sinusoidal axial tests on cylindrical specimens, were carried out at various temperatures (from 0 °C to 50 °C) and frequencies (from 0.1 to 12 Hz). The complex Young's modulus E∗ and the complex Poisson's ratio v∗ were determined through the measurement of axial and transverse strain. We show that when considering E∗, CBTM mixtures may be considered thermo-rheologically simple and the Huet-Sayegh model can be used to simulate the frequency–temperature dependence. On the other hand, when considering v∗ the behavior of CBTM mixtures is very different from that of hot mix asphalt. In particular, its absolute value is almost constant and very close to 0.15. Keywords: Bitumen emulsion, Cold recycling, Complex modulus, Complex Poisson's ratio, Huet-Sayegh mode

Experimental study of bitumen emulsion–cement mortars: mechanical behaviour and relation to mixtures

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Correction to: Experimental study of bitumen emulsion–cement mortars: mechanical behaviour and relation to mixtures

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Diacylglycerol Kinases: Shaping Diacylglycerol and Phosphatidic Acid Gradients to Control Cell Polarity

Diacylglycerol kinases (DGKs) terminate diacylglycerol (DAG) signaling and promote phosphatidic acid (PA) production. Isoform specific regulation of DGKs activity and localization allows DGKs to shape the DAG and PA gradients. The capacity of DGKs to constrain the areas of DAG signaling is exemplified by their role in defining the contact interface between T cells and antigen presenting cells: the immune synapse. Upon T cell receptor engagement, both DGK α and ζ metabolize DAG at the immune synapse thus constraining DAG signaling. Interestingly, their activity and localization are not fully redundant because DGKζ activity metabolizes the bulk of DAG in the cell, whereas DGKα limits the DAG signaling area localizing specifically at the periphery of the immune synapse. When DGKs terminate DAG signaling, the local PA production defines a new signaling domain, where PA recruits and activates a second wave of effector proteins. The best-characterized example is the role of DGKs in protrusion elongation and cell migration. Indeed, upon growth factor stimulation, several DGK isoforms, such as α, ζ, and γ, are recruited and activated at the plasma membrane. Here, local PA production controls cell migration by finely modulating cytoskeletal remodeling and integrin recycling. Interestingly, DGK-produced PA also controls the localization and activity of key players in cell polarity such as aPKC, Par3, and integrin β1. Thus, T cell polarization and directional migration may be just two instances of the general contribution of DGKs to the definition of cell polarity by local specification of membrane identity signaling